Introduction of the novel epitope 2B8(POP) tag

◎ Application of epitope tags for protein research


◎ Present commercialized Epitope tags and antibodies


◎ Journal


◎ Weak points of commercial epitope tags


◎ Applicable to various cell species

     –  No Western blot band was found over total proteins of various plant, animal and microbial cells


◎ 2B8 tag의 성능 우수성 비교 실험


◎ Protein antigen-Antibody Interaction


◎ 기존 에피토프 택과 2B8택의 생화학적 특성

PI change of GFP protein by epitope tagging


 ◎ Current commercial affinity purification method using Epitope tags


◎ Point Mutation of  2B8(RDPLPAFPP) tag


◎ Protein-protein interaction analysis